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. 2020 Sep 4;21(18):6453. doi: 10.3390/ijms21186453

Figure 4.

Figure 4

IRF4 and IRF8 expression is differentially regulated in Stat5b-CA-expressing BMDCs. BMDCs derived from NOD and NOD.CD11cStat5b-CA mice were cultured for 48 h in the absence or presence of GM-CSF (50 ng/mL). (A) Irf4 and Irf8 mRNA expression levels were determined by qRT-PCR using the ΔΔCT method. The data are shown as relative expression compared to untreated BMDCs derived from NOD mice. (B) Representative Western blot analysis of IRF4, IRF8, pStat5, and Stat5b expression. β-actin expression is shown as a gel-loading control. (C–D) Representative flow cytometry analysis of (C) IRF4 and IRF8, and (D) CD11b in CD11c+ BMDCs. Data are shown as the mean ± SEM of at least three independent experiments. The asterisks indicate statistically significant differences determined by one-way ANOVA with Tukey’s post-test. p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), and p < 0.0001 (****).