Figure 2.

AFB₁ exhibits significant cytotoxicity toward IMR-32 cells. (A) The IC50 values of AFB₁ were 6.18 μg/mL and 5.87 μg/mL in IMR-32 cells after 24 h and 48 h of treatment, respectively. (B) Relative LDH release by IMR-32 cells exposed to different concentrations of AFB₁ for 24 h and 48 h. All experiments were repeated three times and the values represent the mean ± SD of three independent experiments. Statistical significance was defined as * p < 0.05, ** p < 0.01, or *** p < 0.001.