Figure 7.

Peripheral cannabinoid receptor 1 (CB1R) antagonist increased oxygen consumption rate (OCR) inhibited by lipolysis blocker. 3T3-L1 adipocytes were treated with AM6545, rimonabant (RIM) with and without Atglinstatin, and isoproterenol (ISO) at 4 and 48 h. OCR was measured in basal conditions or in response to sequential treatment with 2 µM oligomycin, 0.75 µM FFCP (respiratory chain uncoupler), and 1 µM rotenone/antimycin A (inhibitor of respiratory chain complex I and complex III) using Seahorse XF-24 analyzer. (a) Mitochondrial respiration tracing using Seahorse at 4 h after treatment. (b) Parameters calculated from the tracing at 4 h after treatment. (c) Mitochondrial respiration tracing 48 h after treatment. (d) Parameters calculated from the tracing at 48 h after treatment. Data on graphs are presented as mean ± SD of 4 independent rounds of the cells; * p < 0.05 vs. control ** p < 0.01 vs. control.