Figure 6.

Affinity purification of RNA binding proteins (RBPs) of in vitro transcribed RNAs. (A) Table shows SRSF1 binding motif weakening in 1T and 1T2T variants compared to the wild-type construct via their Z-scores. Values correspond to the binding affinity of SRSF1 to each 7-mer [31], and 7-mers with the highest Z-score in analyzed sequences are shown. (B) Western blot analysis after the affinity purification of RNA-binding proteins is shown. Beads alone (Beads) and the HeLa nuclear extract (NE) sample were used as a control. Western blot images and Coomassie gel staining are representative figures in three independent experiments.