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Methodological steps of this research. (A) description of the Pb exposure model and sample size; (B) behavioral tests elected for this investigation: object recognition test and step-down inhibitory avoidance test; (C) samples collection; (D) measurement of Pb levels; (E) analyses of oxidative biochemistry, through pro and antioxidant parameters; (F) proteomic profile performed by mass spectrometry; (G) immunohistochemical analysis for NeuN+ cells density.
