Figure 8.

Kinetics of procollagen III synthesis in poloxamer hydrogel-treated ex vivo cultured human skin explants submitted to 10 s long experimental burn injury. Immunostaining of procollagen III was performed on gel-treated human skin explants harvested at different time points post burn (days 5, 8, 10, and 14). After wound debridement, on day 1, daily treatment with poloxamer p407/p188 hydrogel was applied. No significant change was observed on day 5 in the burn area compared to healthy skin. On day 8, a decreased intensity in procollagen III staining (area delimited by a red dotted line) was observed in the dermal area adjacent to the burnt epidermis. On day 10, this decrease in procollagen III staining intensity was less pronounced but still present until it returned to baseline levels on day 14. Experiments were performed on skin explants derived from three donors. White dashed lines delineate the burnt area. Scale bar: 100 µm.)