Table 3.
The optimized values of collision energy (CE) and declustering potential (DP) of selected Q1 and Q3 MRM transitions for the simultaneous analysis of sterols and tocols using LC-MRM-MS/MS.
| S/No | Q1 (m/z) | Q3 (m/z) | Component Name | DP (V) | EP (V) | CE (eV) | CXP (V) | QL/QT | RT (min) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 430.5 | 165.3 | α-tocopherol (1) | 65 | 10 | 40 | 5 | QT | 14.31 |
| 2 | 430.6 | 205.3 | α-tocopherol (2) | 65 | 10 | 40 | 5 | QL | 14.31 |
| 3 | 397.6 | 147.2 | 24α-ethyl cholesterol (1) | 60 | 10 | 40 | 5 | QT | 20.06 |
| 4 | 397.6 | 161.4 | 24α-ethyl cholesterol (2) | 60 | 10 | 40 | 5 | QL | 20.06 |
| 5 | 383.6 | 161.4 | 24α-methyl cholesterol (1) | 70 | 10 | 40 | 5 | QL | 20.25 |
| 6 | 383.6 | 147.3 | 24α-methyl cholesterol (2) | 70 | 10 | 40 | 5 | QT | 20.25 |
| 7 | 370.4 | 147.7 | Cholesterol (1) | 70 | 10 | 40 | 5 | QT | 20.96 |
| 9 | 370.4 | 161.7 | Cholesterol (2) | 70 | 10 | 40 | 5 | QL | 20.96 |
| 10 | 371.7 | 109.3 | 5-α-Cholestan-3-β-ol (1) | 70 | 10 | 40 | 5 | QT | 21.92 |
| 11 | 371.7 | 135.3 | 5-α-Cholestan-3-β-ol (2) | 70 | 10 | 40 | 5 | QL | 21.92 |
QL: Qualitative, QT: Quantitative, RT: Retention Time.