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. 2020 Sep 13;21(18):6706. doi: 10.3390/ijms21186706

Figure 5.

Figure 5

Cinobufagin suppresses melanoma cell growth by suppressing LEF1. (A) Cinobufagin induces apoptosis. LEF1 stably expressing A375 melanoma cells were incubated with cinobufagin for 72 h. The apoptotic cell population was measured by Annexin-V staining as described in the Materials and Methods section. (B) Cinobufagin attenuates melanoma cell growth dependent on LEF1 expression. LEF1 overexpressing A375 and A2058 melanoma cells were incubated with cinobufagin for 72 h. Cell viability was measured by crystal violet staining. The values represent the mean ± SD (n = 4); * p < 0.05 and ** p < 0.01. (C) LEF1 expression in pBABE-EV or pBABE-LEF1 expressing A375 and A2058 cells. LEF1 protein levels were measured by Western blotting. Red and blue points, mean value of individual sample.