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. 2020 Sep 9;9(9):842. doi: 10.3390/antiox9090842

Table 1.

Primer sets for RT-qPCR.

Gene Primer Sequences NCBI Accession Number
human ACTB 5′-TGGCACCCAGCACAATGAA-3′
5′-CTAAGTCATAGTCCGCCTAGAAGCA-3′
NM_001101.4
human CAT 5′-CCATTATAAGACTGACCAGGGC-3′
5′-AGTCCAGGAGGGGTACTTTCC-3′
NM_001752.3
human GPX1 5′-CAGTCGGTGTATGCCTTCTCG-3′
5′-GAGGGACGCCACATTCTCG-3′
NM_000581.3
human GPX2 5′-CCCCTACCCTTATGATGACC-3′
5′-GTTGATGGTTGGGAAGGTG-3′
NM_002083.3
human GPX3 5′-CGGGGACAAGAGAAGTCG-3′
5′-CCCAGAATGACCAGACCG-3′
NM_002084.4
human GPX4 5′-GAGTTTTCCGCCAAGGACATCGA-3′
5′-GGTCGACGAGCTGAGTGTAGTTT-3′
NM_002085.4
human GSR 5′-ATGATCAGCACCAACTGCAC-3′
5′-CGACAAAGTCTTTTTAACCTCCTT-3′
NM_000637.4
human GSS 5′-AAGACACTCGTGATGAACAAG-3′
5′-AGAGGAATGACAAATACAGAGGAT-3′
NM_000178.3
human HO-1 5′-ATGGCCTCCCTGTACCACATC-3′
5′-TGTTGCGCTCAATCTCCTCCT-3′
NM_002133.2
human KEAP1 5′-ATTGGCTGTGTGGAGTTGC-3′
5′-CAGGTTGAAGAACTCCTCTTGC-3′
NM_012289.3
human NQO-1 5′-ATCCTGCCGAGTCTGTTCTG-3′
5′-AGGGACTCCAAACCACTGC-3′
NM_000903.2
human Nrf2 5′-CTTTTGGCGCAGACATTCC-3′
5′-AAGACTGGGCTCTCGATGTG-3′
NM_006164.4
human PRDX1 5′-AGGCCTTCCAGTTCACTGAC-3′
5′-CAGGCTTGATGGTATCACTGC-3′
NM_002574.3
human SOD1 5′-TGGTTTGCGTCGTAGTCTCC-3′
5′-CTTCGTCGCCATAACTCGCT-3′
NM_000454.4
human SOD2 5′-GGAAGCCATCAAACGTGACTT-3′
5′-CCCGTTCCTTATTGAAACCAAGC-3′
NM_000636.3
human SOD3 5′-GGTGCAGCTCTCTTTTCAGG-3′
5′-AACACAGTAGCGCCAGCAT-3′
NM_003102.2
human TXN 5′-GCCTTTCTTTCATTCCCTCTC-3′
5′-GCTTTTCCTTATTGGCTCCAG-3′
NM_003329.3
human TXNRD1 5′-TGTTGGAGCATCCTATGTCG-3′
5′-TCAAATCCTCTAAGAAGAATGGACC-3′
NM_182729.2
human xCT 5′-TCCTGCTTTGGCTCCATGAACG-3′
5′-AGAGGAGTGTGCTTGCGGACAT-3′
NM_014331.3
mouse Actb 5′-GGCTGTATTCCCCTCCATCG-3′
5′-CCAGTTGGTAACAATGCCATGT-3′
NM_007393.5
mouse Ho-1 5′-ACAGAGGAACACAAAGACCAG-3′
5′-GTGTCTGGGATGAGCTAGTG-3′
NM_010442.2
mouse Il-1β 5′-GCAACTGTTCCTGAACTCAACT-3′
5′-ATCTTTTGGGGTCCGTCAACT-3′
NM_008361.4
mouse iNos 5′-GGCAGCCTGTGAGACCTTTG-3′
5′-TGCATTGGAAGTGAAGCGTTT-3′
NM_010927.4
mouse Nrf2 5′-CCCAGCAGGACATGGATTTGA-3′
5′-AGCTCATAGTCCTTCTGTCGC-3′
NM_010902.4