Figure 2.

BSA blocking on different types of 96-well plates. BSA was used to prevent non-specific adhesion of platelets to the plastic. To find the optimum conditions, 3 types of 96-well plates (Invitrogen™—442404; Greiner Bio-one—655,180 and Falcon—353072) were blocked with different concentrations of BSA (0.00075 to 4% in 50 µL) for 1 hour at 37 °C. Next, human washed platelets (8 × 10⁴/µL in 100 µL) were added, followed by incubation for 1 h at 37 °C. Non-adherent platelets were removed, and adherent platelets were incubated with BCECF-AM (4 µg/mL in 50 µL) for 30 min at 37 °C. Fluorescence intensity was measured using a plate reader (VictorX, PerkinElmer, Waltham, MA, USA). Values were compared to the control condition—without BSA—by one-way ANOVA followed by Dunnett’s post hoc test (**** p < 0.0001, data are mean ± SEM; n = 4).