Table 2.
Previous studies using BCECF-AM to measure platelet adhesion.
| Year | Type of Plate | ECM Concentration | Blocking BSA | Platelet Concentration | Dye Concentration | Type of Measurement | Ref. |
|---|---|---|---|---|---|---|---|
| 1996 | 96-well (N/A) |
Collagen (100 μg/mL) | 0.35% | 300,000/μL | Pre-labeled platelets with 2 μM BCECF-AM at 37 °C, 30 min | Plate reader without lysing platelets | [48] |
| 1997 | 96-well (Falcon) |
Collagen-I or Fibrinogen (100 μg/mL) |
0.0005% | 375,000/μL | Pre-labeled platelets with 6 mM BCECF-AM at 37 °C, 30 min | Plate reader after lysing platelets | [49] |
| 1997 | 96-well (Costar) |
Fibronectin or Vitronectin (0.05 to 1.5 μg/well) | 0.50% | 300,000/μL | Pre-labeled platelets with 5 μM BCECF-AM at 37 °C, 40 min | Plate reader without lysing platelets | [50] |
| 1997 | 96-well (Costar) |
Fibronectin, Vitronectin, vWF, laminin (1 μg/well) or collagen-IV (5 μg/well) |
0.50% | 300,000/μL | Pre-labeled platelets with 5 μM BCECF-AM at 37 °C, 40 min | Plate reader without lysing platelets | [51] |
| 1998 | 96-well (N/A) |
Fibrinogen (0.01, 0.1 and 2 μg/well) | N/A | 400,000/μL | Pre-labeled platelets with 12 μM BCECF-AM at 37 °C, 30 min | Plate reader without lysing platelets | [52] |
| 1999 | 96-well (Immulon-2) |
Fibrinogen (1 ng to 2 μg/well) | N/A | 4,000,000/μL | Pre-labeled platelets with 6 μM BCECF-AM at 37 °C, 30 min | Plate reader without lysing platelets | [53] |
| 1999 | 96-well (Costar) |
Fibronectin, Vitronectin, vWF or Laminin (120 μg/mL) | 0.50% | 300,000/μL | Pre-labeled platelets with 5 μM BCECF-AM at 37 °C, 40 min | Plate reader without lysing platelets | [54] |
| 2008 | 96-well (N/A) |
Collagen or Fibrinogen (50 μg/mL) |
1% | N/A | Pre-labeled platelets with BCECF-AM for 40 min (temperature and concentration N/A) | Microscopy | [55] |
| 2010 | 96-well (N/A) |
Collagen or Fibrinogen(50 μg/mL) | 1% | N/A | Pre-labeled platelets with BCECF-AM for 30 min (temperature and concentration N/A) | Plate reader without lysing platelets | [56] |
| 2018 | 96-well (N/A) |
Collagen (10 μg/mL) | 0.5% or 0.05% | N/A | Pos-labelling platelets with 12 μM BCECF-AM (incubation time and temperature N/A) | Plate reader after lysing platelets | [57] |
| 2019 | 96-well (N/A) |
Fibrinogen (100 μg/mL) |
5% | 200,000/μL | Pre-labeled platelets with BCECF-AM at 37 °C for 30 min (concentration N/A) | Plate reader after lysing platelets | [58] |