Table 1.
Possible Amino Acids at Positions in or Around Cleavage Site for Specific Proteases.
| Protease | P4 Position | P3 Position | P2 Position | P1 Position | P1′ Position | P2′ Position | P3′ Position | P4′ Position |
|---|---|---|---|---|---|---|---|---|
| Alcalase | Gly, Pro, Ala, Val, Leu, Met, Phe, Tyr, Ser, Thr, Asn, Glu, His | Gly, Ala, Val, Leu, Phe, Ser, Thr, Asn, Gln, Asp, Glu, Lys, Arg, His | Gly, Pro, Ala, Val, Leu, Ile, Phe, Tyr, Ser, Thr, Asn, Gln, Glu, Arg, His | Ala, Val, Leu, Met, Phe, Tyr, Ser, Asn, Gln, Glu, Lys, Arg, His | Gly. Ala, Val, Leu, Met, Phe, Tyr, Ser, Thr, Gln, His | Gly, Pro, Ala, Val, Leu, Ile, Phe, Tyr, Ser, Thr, Gln, Glu, Arg, His | Gly, Pro, Ala, Val, Leu, Met, Phe, Tyr, Ser, Thr, Asn, Lys, Arg, His | Gly, Pro, Ala, Val, Leu, Tyr, Ser, Thr, Cys, Asn, Gln, Asp, Glu, Lys, His |
| Stem Bromelain | Pro, Ala, Leu, Ile, Phe, Tyr, Ser, Thr, Cys, Glu, Arg, His | Gly, Pro, Ala, Val, Leu, Phe, Ser, Thr, Lys, Arg, His | Gly, Pro, Val, Leu, Phe, Tyr, Ser, Thr, Asn, Arg, His | Gly, Ala, Val, Leu, Phe, Tyr, Ser, Thr, Asn, Gln, Arg | Gly, Val, Leu, Ile, Phe, Tyr, Ser, Thr, Asn, Gln, Asp, Glu, Lys, His | Pro, Ala, Val, Leu, Tyr, Ser, Thr, Asn, Gln, Asp, Glu, Lys, His | Gly, Pro, Val, Leu, Phe, Ser, Thr, Cys, Asn, Gln, Asp, Glu, Arg, His | Gly, Pro, Ala, Val, Leu, Phe, Tyr, Ser, Thr, Asp, Glu, Lys, Arg, His |
| Ficin | Pro, Leu, Ser, Glu, Lys, Arg, His | Gly, Ala, Val, Leu, Ile, Phe, Thr, Arg, His | Gly. Val, Leu, Phe, Thr, Lys | Gly, Leu, Phe, Tyr, Ser, Lys, Arg, His | Leu, Phe, Tyr, Ser, Thr, Lys, His | Val, Leu, Tyr, Thr, Asn, Lys, His | Pro, Ala, Val, Leu, Ser, Thr, Glu, Lys | Gly, Pro, Val, Asn, Asp, Glu, Lys |
| Papain | Gly, Pro, Ala, Val, Leu, Ile, Phe, Tyr, Ser, Thr, Cys, Asn, Asp, Glu, Arg, His | Gly, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, Ser, Gln, Asp, Glu, Lys, Arg, His | All 20 Canonical AA | All 20 Canonical AA | Gly, Ala, Val, Leu, Ile, Met, Phe, Tyr, Ser, Thr, Asn, Gln, Asp, Glu, Lys, His | Gly, Pro, Ala, Val, Leu, Ile, Phe, Tyr, Ser, Thr, Cys, Asn, Gln, Glu, Arg, His | Gly, Pro, Ala, Val, Leu, Phe, Tyr, Ser, Cys, Glu, Lys, Arg, His | Gly, Pro, Ala, Val, Leu, Ile, Phe, Tyr, Ser, Thr, Asn, Asp, Glu, Lys, Arg, His |
| Thermolysin | All 20 Canonical AA | All 20 Canonical AA | All 20 Canonical AA | All 20 Canonical AA | Gly, Pro, Ala, Val, Leu, Ile, Met, Phe, Tyr, Trp, Ser, Thr, Asn, Gln, Asp, Glu, Lys, His | All 20 Canonical AA | All 20 Canonical AA | All 20 Canonical AA |
AA = Amino Acids.