Table 3.
Enzyme Scheme | Time (min) | ABTS Radical Scavenging Activity (Ascorbic Acid Equivalent, μM) |
Iron Chelating Activity (EDTA Equivalent, μM) |
ORAC (Trolox Equivalent, μM) |
---|---|---|---|---|
Thermolysin + MBA | 0 | 685.0 ± 333.8 | 3034.8 ± 216.4 | 127 ± 19 |
5 | 632.7± 288.6 | 5334.9 ± 45.3 | 125 ± 21 | |
10 | 683.2 ± 296.1 | 5337.8 ± 38.3 | 115 ± 17 | |
25 | 449.7 ± 301.4 | 5328.1 ± 17.4 | 98 ± 39 | |
45 | 511.0 ± 255.7 | 5329.2 ± 35.4 | 116 ± 17 | |
60 | 493.9 ± 297.7 | 5336.1 ± 26.2 | 127 ± 17 | |
240 | 646.2 ± 324.7 | 5161.7 ± 280.2 | 127 ± 17 | |
0 | 513.7 ± 230.4 | 3005.5 ± 248.0 | 122 ± 17 | |
15 | 533.5 ± 332.0 | 472.4 ± 227.4 | 126 ± 8 | |
Pepsin + MBA | 25 | 535.3 ± 328.0 | 529.4 ± 234.1 | 96 ± 20 |
45 | 540.8 ± 307.3 | 500.2 ± 173.3 | 99 ± 35 | |
60 | 477.7 ± 316.6 | 490.7 ± 300.4 | 122 ± 12 | |
90 | 638.1 ± 340.2 | 594.3 ± 108.6 | 107 ± 42 | |
120 | 495.7 ± 342.3 | 434.3 ± 230.1 | 101 ± 46 | |
0 | 487.6 ± 338.4 | 448.5 ± 240.1 | 145 ± 38 | |
15 | 450.2 ± 283.0 | 4924.6 ± 119.4 | 105 ± 5 | |
25 | 474.1 ± 245.5 | 4901.0 ± 95.0 | 97 ± 31 | |
Pepsin-Pancreatin + MBA | 45 | 559.7 ± 197.0 | 4886.9 ± 182.8 | 121 ± 17 |
60 | 456.9 ± 260.3 | 4884.4 ± 105.6 | 125 ± 16 | |
90 | 552.9 ± 256.4 | 4794.9 ± 16.5 | 124 ± 16 | |
120 | 581.3 ± 242.9 | 4843.5 ± 155.5 | 127 ± 15 |
MBA = Mung Bean Albumin; 1.0 mg/mL of EDTA is equivalent to 3421.8 μM; 1.5 mg/mL of EDTA is equivalent to 5132.8 μM; 0.15 mg/mL of ascorbic acid is equivalent to 851.7 μM; 1.0 mg/mL of ascorbic acid is equivalent to 5677.9 μM.