Figure 3.

Effects of aronia bioactive fraction (ABF) on the lipopolysaccharide (LPS)-induced mRNA expression of interleukin (IL)-6 (A), IL-8 (B) and interleukin 1 beta (IL-1β) (C) regulated upon activation, normal T cell expressed and presumably secreted (RANTES) (D), tumor necrosis factor (TNF)-α (E) and cyclooxygenase-2 (COX-2) (F). BEAS-2B cells were pretreated with ABF (10 and 25 μg/mL) 6 h before treatment with LPS (1 μg/mL). After incubation for 6 h, the mRNA was evaluated by quantitative real time-polymerase chain reaction. The values are presented as means ± standard deviation (SD) of three independent experiments. * p < 0.1, ** p < 0.05, *** p < 0.01 indicate significant differences as compared to the LPS-treated cells.