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. 2020 Sep 4;21(18):6482. doi: 10.3390/ijms21186482

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunoblot analysis of activation of downstream substrates in TGF-β1/2 knockdown RGC-5 cells with hyperglycemia. Immunoblot analysis of antioxidation pathway-associated proteins (Nrf2, Keap1, HIF-1α, ALDH3A1, and HO-1) in 5.5 mM, 25 mM, 50 mM, and 100 mM glucose supplemented medium. The protein expression values were quantified in relation to the loading control. Data are represented as means ± SEM. **, p < 0.01; ***, p < 0.001; ns, non-significant, when compared to the 5.5 mM group (n = 3).

Immunoblot analysis of activation of downstream substrates in TGF-β1/2 knockdown RGC-5 cells with hyperglycemia. Immunoblot analysis of antioxidation pathway-associated proteins (Nrf2, Keap1, HIF-1α, ALDH3A1, and HO-1) in 5.5 mM, 25 mM, 50 mM, and 100 mM glucose supplemented medium. The protein expression values were quantified in relation to the loading control. Data are represented as means ± SEM. **, p < 0.01; ***, p < 0.001; ns, non-significant, when compared to the 5.5 mM group (n = 3).