Figure 2.

A scheme of proteomic screening and identification of amyloids (PSIA) method for amyloid screening. The pellet fraction of a homogenized sample, that is resistant to sodium dodecyl sulfate or sarcosyl is analyzed by high performance liquid chromatography followed by mass-spectrometry. Individual candidate proteins, identified by PSIA, can be further investigated in vivo and in vitro using conventional approaches such as circular dichroism spectrum, X-ray fiber diffraction, ability to bind Congo red dye with green/yellow birefringence in polarized light, etc.