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. 2020 Sep 22;8(9):370. doi: 10.3390/biomedicines8090370

Figure 5.

Figure 5

Effect of AR siRNA on cell migration, proliferation, and apoptosis-related markers in Brassicasterol treated LNCaP cells. LNCaP cells were transfected with AR siRNA for 24 h and were incubated in the presence or absence of brassicasterol (10 μM) for 24 h. (A) A wound-healing assay assessed cell migration. Bar graph represents the quantification of cell migration, present as a percentage of control of siRNA. (*) p < 0.05, (**) p < 0.01 (in comparison to 24 h control of siRNA) (##) p < 0.01 (in comparison to 0h control). (B) The cell lysates were prepared and subjected to Western blotting to determine the expression of AR, PSA, p-AKT, AKT, E-cadherin, vimentin, PARP, and β-actin. Bar graphs represent the quantification of interest protein related to β-actin, present as a fold change of control of siRNA. (*) p < 0.05, (***) p < 0.01 (in comparison to control).