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. 2020 Sep 22;8(9):370. doi: 10.3390/biomedicines8090370

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of AKT siRNA on cell migration- and proliferation-related makers in Brassicasterol treated prostate cancer cells. (A–B) LNCaP cells were transfected with AKT siRNA for 48 h and were incubated in the presence or absence of brassicasterol (10 μM) for 24 h. (A) The cells growth was determined using Cellomax kit. The graph showed quantitative cell growth. (**) p < 0.01 and (***) p < 0.001 compared with control. (B) The cell lysates were prepared and subjected to Western blotting to determine the expression of AR p-AKT, AKT, E-cadherin, vimentin, and β-actin. Bar graphs represent the quantification of interest protein related to β-actin, present as a fold change of control of siRNA. (*) p < 0.05, (**) p < 0.01, and (***) p < 0.001 (in comparison to control of siRNA). (C) The cells were evaluated to cell migration by wound healing assay. Bar graph represents the quantification of cell migration, present as a percentage of control of siRNA. (*) p < 0.05, (**) p < 0.01 (in comparison to 24h control of siRNA) (##) p < 0.01 (in comparison to 0 h control). (D–F) PC-3 cells were transfected with AKT siRNA for 48 h and were incubated in the presence or absence of Brassicasterol (10 μM) for 24 h. (D) The cells were measured cell growth by Cellomax kit. The graph showed quantitative cell growth. (*) p < 0.05, and (***) p < 0.001 compared with control. (E) The cell lysates were prepared and subjected to Western blotting to determine the expression of p-AKT, AKT, E-cadherin, vimentin, and β-actin. Bar graphs represent the quantification of interest protein related to β-actin, present as a fold change of control of siRNA. (*) p < 0.05, (**) p < 0.01, and (***) p < 0.001 (in comparison to control of siRNA) (F) The cells were evaluated to cell migration by wound healing assay. Bar graph represents the quantification of cell migration, present as a percentage of control of siRNA. (*) p < 0.05(in comparison to 24 h control of siRNA) (##) p < 0.01 (in comparison to 0 h control).

Effect of AKT siRNA on cell migration- and proliferation-related makers in Brassicasterol treated prostate cancer cells. (A–B) LNCaP cells were transfected with AKT siRNA for 48 h and were incubated in the presence or absence of brassicasterol (10 μM) for 24 h. (A) The cells growth was determined using Cellomax kit. The graph showed quantitative cell growth. (**) p < 0.01 and (***) p < 0.001 compared with control. (B) The cell lysates were prepared and subjected to Western blotting to determine the expression of AR p-AKT, AKT, E-cadherin, vimentin, and β-actin. Bar graphs represent the quantification of interest protein related to β-actin, present as a fold change of control of siRNA. (*) p < 0.05, (**) p < 0.01, and (***) p < 0.001 (in comparison to control of siRNA). (C) The cells were evaluated to cell migration by wound healing assay. Bar graph represents the quantification of cell migration, present as a percentage of control of siRNA. (*) p < 0.05, (**) p < 0.01 (in comparison to 24h control of siRNA) (##) p < 0.01 (in comparison to 0 h control). (D–F) PC-3 cells were transfected with AKT siRNA for 48 h and were incubated in the presence or absence of Brassicasterol (10 μM) for 24 h. (D) The cells were measured cell growth by Cellomax kit. The graph showed quantitative cell growth. (*) p < 0.05, and (***) p < 0.001 compared with control. (E) The cell lysates were prepared and subjected to Western blotting to determine the expression of p-AKT, AKT, E-cadherin, vimentin, and β-actin. Bar graphs represent the quantification of interest protein related to β-actin, present as a fold change of control of siRNA. (*) p < 0.05, (**) p < 0.01, and (***) p < 0.001 (in comparison to control of siRNA) (F) The cells were evaluated to cell migration by wound healing assay. Bar graph represents the quantification of cell migration, present as a percentage of control of siRNA. (*) p < 0.05(in comparison to 24 h control of siRNA) (##) p < 0.01 (in comparison to 0 h control).