Figure 4.

Priming of elicitor-induced NADPH-oxidase-mediated ROS production in plants pre-treated with inulin and LOS (A,B). ROS production in leaf disks in response to (A) 100 nM flg22 or (B) 0.2 mg/mL OGs 24 h after pre-treatment with 5 g/L fructan. Diphenyleneiodonium (DPI) was added to 5 µM final concentration for NADPH-oxidase inhibition. The graph represents the mean ± SE (n ≥ 8). (C,D) Absence of a ROS burst in leaf disks in response to inulin and LOS treatment, as compared to BABA, OGs, and H₂O control treatments with no elicitor. Arabidopsis leaf disks were treated with different concentrations of (C) inulin and (D) LOS and compared to OGs at 0.2 mg/mL and BABA at 1mM. Values represent the mean ± SE (n = 16) and expressed as relative light units (RLU).