Figure 6.

Effect of fructan treatment on H₂O₂ content and H₂O₂ scavenging enzymes in the leaves after treatment and B. cinerea infection. Arabidopsis plants treated with 5 g/L inulin or LOS followed by B. cinerea infection 72 h later were analyzed for (A) H₂O₂ content, (B) catalase activity, (C) ascorbate peroxidase activity and (D) guaiacol peroxidase activity. Bars represent the mean ± SE of 6 biological replicates. Statistical significance is indicated by different letters (a–c) within the same timepoint (p < 0.05) with asterisks (*** p < 0.001) between time points and is based on two-way ANOVA, followed by Tukey’s multiple comparison test and adjusted p values. Experiments were repeated three times with consistent results. (E) Representative leaves analyzed for H₂O₂ distribution by DAB staining 24 h after infection.