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Effect of the temperature on the secondary structure of RiLK1. Far-UV CD spectra of the peptide (0.1 mg/mL) were acquired in 10 mM Tris-HCl buffer pH 7.0 in the presence of 3 mM SDS at three different temperatures (4 °C, 25 °C, and 90 °C) after 24 h incubation.
