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. 2020 Sep 22;21(18):6952. doi: 10.3390/ijms21186952

Figure 9.

Figure 9

Covalent immobilization of RiLK1 on polypropylene (PP) disks. (A) Time course of the coupling reactions of RiLK1 on PP surface determined by RP-HPLC on μBondapak C18 column for the immobilization yield (%) measurement. The PP disks, pre-activated by corona treatment, were incubated for different times in a solution of RiLK1 (50 µM) in DMSO in presence of molecular sieves. After each coupling reaction, the solutions were recovered and analyzed by RP-HPLC. A reference sample (t = 0) prepared with the same peptide solution placed in contact with PP films without adding the molecular sieves was used as control. The chromatogram is representative of three independent experiments. The chromatographic profile in green represents the release analysis of RiLK1 from functionalized PP disks performed by RP-HPLC on C18 column after 24 h incubation at 4 °C in mozzarella brine. A solution of RiLK1 (50 μM) was used as control. (B) Stereomicroscope photographs of PP disks without (CTRL) and with the peptide (RiLK1) after the immobilization procedure. The microscope images are representative of three independent experiments performed in triplicate. Bar is equal 0.5 mm.