Table 2.
Secondary structure contents of RiLK1 in the absence or presence of SDS (3 mM) determined by BeStSel (Beta Structure Selection) and DichroWeb server.
| Incubation Time | BeStSel | DichroWeb CONTIN-LL |
||||
|---|---|---|---|---|---|---|
| α-Helix | β-Sheet | Random | α-Helix | β-Sheet | Random | |
| Without SDS | 1.2% | 47.3% | 51.5% | 4% | 35% | 61% |
| t = 0 with SDS | 7.4% | 51.4% | 41.3% | 22% | 38% | 40% |
| t = 5 min with SDS | 4.4% | 53.4% | 42.2% | 22% | 42% | 36% |
| t = 30 min with SDS | 11% | 48.7% | 40.3% | 25% | 38% | 37% |
| t = 1 h with SDS | 9.8% | 51.1% | 39.1% | 23% | 38% | 39% |
| t = 5 h with SDS | 6.7% | 54% | 39.3% | 19% | 43% | 38% |
| t = 6 h with SDS | 8% | 52% | 40.1% | 19% | 44% | 37% |
| t = 24 h with SDS | 8.4% | 53.6% | 38% | 29% | 47% | 24% |