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. 2020 Sep 4;21(18):6455. doi: 10.3390/ijms21186455

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Study design workflow. Adipose tissue-derived mesenchymal stem cells (AT-MSCs) and bone marrow-derived mesenchymal stem cells (BM-MSCs) were first characterized and then used for conditioned media studies with human tongue squamous carcinoma (HSC-3) cells. Cells growing in conditioned media were analyzed for long interspersed nuclear element-1 (LINE-1) methylation and changes in expression of microenvironment remodeling genes. AT-MSCs, HSC-3 cells, dysplastic oral keratinocyte (DOK) cells, and normal oral fibroblasts (NOFs) were used in a co-culture system for invasion, proliferation and migration studies.