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. 2020 Sep 4;10(9):674. doi: 10.3390/diagnostics10090674

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of cancer stemness markers in UBE2C-knockdown OSCC cells. (A) SAS cells. (B) Ca9-22 cells. The expression levels of the ALDH1/A1, ALDH1/A2, CD44, ABCG2, CD166 and EpCAM in cells transfected with scrambled s siRNA (5 nM, siCtrl) or siRNAs against UBE2C (5 nM, siUBE2C) were detected by real time polymerase chain reaction (RT-PCR) and GAPDH was used as an internal control. The quantitative results are expressed as the mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. nontargeting control siRNA (siCtrl).