Figure 3.

Complement regulator properdin changed complement mRNA expression in stressed hpRPE cells. (A) H₂O₂ stressed hpRPE cells were treated with properdin (CFP) for 4 h. mRNA expression of main complement components C1Q, C3, C5, and CFB (black) as well as complement receptors C3AR, C5AR1, and CD11B (grey) was decreased in comparison to stressed cells (dotted line). CFD (black) and complement regulators CFI and CFH (white) mRNA expression was increased in stressed, properdin-treated cells compared to stressed cells only. Data from donors 1–4 are presented. Mean with standard deviation is shown. * p < 0.05, ** p < 0.01 unpaired t-test with Welch‘s correction of stressed versus stressed, properdin-treated cells. (B) H₂O₂ stressed hpRPE cells of donor 1 were treated with properdin for 4 h (dark blue) or 24 h (light blue). mRNA Expression of complement components and receptors was reversed after 24 h compared to 4 h. Mean with standard deviation is shown. * p < 0.05, ** p < 0.01 unpaired t-test with Welch‘s correction of 4 h versus 24 h properdin treatment.