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. 2020 Sep 9;21(18):6595. doi: 10.3390/ijms21186595

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Determination of optimal microsphere-protein molar ratio for microsphere-based assay. Serum from a COVID-19 patient was used. The mean fluorescent intensity at different microsphere-protein molar ratio are shown for (A) nucleoprotein (NP) and (B) receptor binding domain (RBD), and the corresponding stacked histogram of selected microsphere-protein molar ratio are shown in (C) NP (1:2) and (D) RBD (1:1). Experiment was performed in triplicate with serum specimen collected from 3 different COVID-19 patients and a representative graph is shown. Error bar represents the standard error of mean from 3 replicates.