Figure 2.

Cytotoxicity of platinum compounds in A498 cells. (A): Relative cell count (RCC) after treatment with cisplatin, carboplatin or oxaliplatin. A498 cells were incubated for 1 h with cisplatin (10, 20, 40, 80 µM), carboplatin (80, 160, 320, 500 µM) or oxaliplatin (20, 40, 80, 160 µM). After 72 h postcultivation, cell count was determined. RCC was obtained by normalization to the untreated control. The mean values are shown of three independent experiments ± standard deviation. (B)/(C): Colony formation ability (CFA) of A498 cells after treatment with (B) cisplatin or oxaliplatin or (C) carboplatin. Cells were incubated for 1 h with cisplatin (5, 10, 15, 20 µM), oxaliplatin (5, 10, 15, 20 µM) or carboplatin (50, 100, 150, 200 µM). Afterwards, cells were cultivated for 12 days for colony formation. Colonies were counted and the treated samples were normalized to the untreated control. The mean values are shown of three independent experiments ± standard deviation.