Figure 4.

Gene expression of IL-8 in A498 cells after incubation with cisplatin, carboplatin or oxaliplatin. Cells were treated for 1 h with cisplatin (10, 20, 50 µM), carboplatin (50, 100, 200, 300, 400, 500 µM) or oxaliplatin (20, 50, 100, 150 µM), followed by a postcultivation of 24 h. Afterwards, gene expression was analyzed using high-throughput RT qPCR. The log2 mean values are shown of three independent experiments normalized to the untreated control whereby the control equals 0 ± standard deviation. The data for cisplatin are taken from [8].