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. 2020 Sep 21;21(18):6928. doi: 10.3390/ijms21186928

Figure 8.

Figure 8

Analysis of apoptotic and necrotic cells after incubation of A498 cells with cisplatin, carboplatin or oxaliplatin. Cells were incubated for 1 h with cisplatin (50 µM), carboplatin (200, 500 µM) or oxaliplatin (20, 50 µM) followed by postcultivation of 24, 48, 72 or 96 h. To distinguish between apoptotic and necrotic + late apoptotic cells, cells were stained with Annexin V-FITC and propidium iodide (PI). Cell cycle distribution was then analyzed by flow cytometry. The mean values are shown of three independent experiments ± standard deviation. The data for cisplatin are taken from [8].