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. 2020 Sep 13;21(18):6710. doi: 10.3390/ijms21186710

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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DNA binding ability of SNBP obtained from: control 1 (a); son (b) and father (c) analyzed by EMSA on 1% agarose gel. Bands on gel representing the state of pGEM3 plasmid DNA incubated in a w/w ratio with increasing amount of SNBP from samples containing: CP/Hr (a); nCP/Hr (b); only–H (c). Panel a from supplementary material of Lettieri et al., 2020 [11]. L-group: low impact area group; EMSA: electrophoresis mobility shift Assay; SNBP: sperm nuclear basic proteins; CP/Hr: canonical protamines/histones ratio; nCP/Hr: not canonical protamines/histones ratio; only-H: only histones and other basic proteins.