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. 2020 Sep 9;8(9):338. doi: 10.3390/biomedicines8090338

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Inhibition of triple-negative breast cancer (TNBC) progression by blocking microtubule acetylation. (A) Representative images showing acetyl-α-tubulin expression in various subtypes of human breast cancer. Scale bar, 500 µm. The intensity was analyzed using Aperio Image Scope software. (B) Expression of microtubule acetylation in breast cancer cell lines (upper panel) categorized according to the subtype (lower panel). (C) Confirmation of acetyl-α-tubulin expression in alpha-tubulin acetyltransferase 1 (αTAT1) Knockout (KO) MDA-MB-231 cell lysates. (D) Anchorage-independent growth assays using αTAT1 KO MDA-MB-231 cells for 3 weeks. The number of colonies was quantified using ImageJ software. Scale bar, 1 mm. ***, p ≤ 0.001. (E) Mock and αTAT1 KO MDA-MB-231 cells were injected into the mammary fat pad of non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice (n = 5, each). *, p ≤ 0.05; ***, p ≤ 0.001.