Table 3.
Summary of EV protein markers annotated against their Biological Processes (GO), KEGG Pathways, Reactome Pathways, and Cellular Components (GO).
| Specific Group | N * | Biological Processes (GO) | Cellular Components (GO) | KEGG Pathways | Reactome Pathways |
|---|---|---|---|---|---|
| NCI-H23 | 8 | NA | Extracellular region (ANXA5|ICAM1|MFGE8 **, SDC4, SPOCK2, TFPI2, TUBA4A, COL6A1,), FDR = 6.07 × 10−6 | ECM-receptor interaction (COL6A1|SDC4), FDR = 0.0132 | Extracellular matrix organization (COL6A1|SDC4, ICAM1), FDR = 0.0227 |
| A549 | 21 | Cellular component organization NRP1|RAC1|RACGAP1|KIF23|KIF4A|PDCD6IP|TSG101|VPS28, HSPG2|COL18A1|ITGA3 (ADAM10, CNP, CNTN1, MARK2, PACSIN2, PTGFRN, SLC2A1), FDR = 2.78 × 10−5 |
Flemming body (RACGAP1|KIF23|KIF4A|PDCD6IP|TSG101, SLC2A1), FDR = 5.11 × 10−6 | NA | Vesicle-mediated transport (RAC1|RACGAP1|KIF23|KIF4A|TSG101|VPS28, PACSIN2), FDR = 0.00046 |
| HTC116 | 2 | NA | Melanosome (ITGB3, STOM), FDR = 0.0029 | NA | NA |
| Caco-2 | 7 | Extracellular matrix organization (COL18A1|FN1|VTN, SPINT2), FDR = 8.8 × 10−4 | Extracellular exosome (FN1|PROM1), FDR = 0.0057 | NA | Integrin cell surface interactions (COL18A1|VTN), FDR = 0.0123 |
| HT29 | 6 | Actomyosin contractile ring assembly (KIF23|RACGAP1), FDR = 1.7 × 10−4 | Central spindlin complex (KIF23|RACGAP1), FDR = 6.42 × 10−5 | MicroRNAs in cancer (KIF23, ST14), FDR = 8.7 × 10−4 | Kinesins (KIF23|RACGAP1), FDR = 0.0036 |
| Lung | 6 | Negative regulation of epidermal growth factor receptor signaling (EPS15|TSG101, GPRC5A), FDR = 9.26 × 10−5 | ESCRT I complex (TSG101|VPS28), FDR = 3.5 × 10−4 | Endocytosis (EPS15|TSG101|VPS28) FDR = 3.77 × 10−5 | ESCRT, (TSG101|VPS28), FDR = 2.9 × 10−4 |
| Colon | 4 | NA | Extracellular region (C4B, COL6A2, DMBT1, TUBA4A), FDR = 0.02 | NA | NA |
| Universal | 11 | Vesicle-mediated transport (CDC42|FN1|HSPG2|ITGB1|ITGB3|TLN1, MVP, TUBA4A), FDR = 4.2 × 10−4 | Melanosome (ITGB1|ITGB3, CNP, SLC2A1), FDR = 6.43 × 10−5 | Rap1 signaling pathway (CDC42|GNAI2|ITGB1|ITGB3|TLN1), FDR = 4.75 × 10−6 | Platelet activation, signaling and aggregation (CDC42|GNAI2|ITGB3|TLN1, TUBA4A), FDR = 3.65 × 10−5 |
* EV protein total number: ** Proteins enriched in interactions according to the STRING interaction analysis are shown in bold and delimited by bars. NA: not available.