Figure 2.

2 Hz EA treatment reversed the SNI-induced up-regulation of brain-derived neurotrophic factor (BDNF) in the spinal cord. (A) Relative levels of BDNF mRNA expression (n = 4 per group). (B) Western blotted band of BDNF. (C) The relative level of BDNF protein expression (n = 9 per group). SNI increased BDNF protein expression in the spinal cord, which was attenuated by 2 Hz EA treatment. Samples were collected from the spinal cord of L4–L6 segments on day 14. ** p < 0.01, *** p < 0.001, compared with the Sham group. ^▲▲ p < 0.01, ^▲▲▲ p < 0.001, compared with the SNI group. All data were expressed as mean ± SEM, one-way ANOVA was followed by the Newman–Keuls post-hoc test.