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. 2020 Sep 22;21(18):6963. doi: 10.3390/ijms21186963

Figure 4.

Figure 4

Effect of limonoids on the cell viability (A) and LPS-induced pro-inflammatory cytokine production (B–D). RAW 264.7 cells pretreated with compounds 1–13 (1, 10, and 100 µM) for 2 h were stimulated with 1 µg/mL LPS for 18 h. Cell viability was determined via MTT assay. The amount of IL-1β (A), IL-6 (B), and TNF-α (C) in the culture medium was measured with ELISA kits. The values are expressed as mean ± standard deviation of three individual experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, compared to the control group.