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. 2020 Sep 21;9(9):895. doi: 10.3390/antiox9090895

Figure 4.

Figure 4

Effect of ethyl acetate extract of Ribes diacanthum Pall (RDP) on apoptotic bodies and mitochondrial membrane potential in HT-22 cells by flow cytometry. (A) Apoptotic bodies and (B) mitochondrial membrane potential in (a) vehicle control; (b) glutamate 5 mM; (c) glutamate 5 mM + RDP 50 μg/mL; (d) glutamate 5 mM + RDP 10 μg/mL; (e) glutamate 5 mM + RDP 25 μg/mL, and (f) glutamate 5 mM + RDP 5 μg/mL. HT-22 cells were seeded in 60-mm dishes for 24 h and then treated with or without RDP (0–50 µg/mL) for 30 min before glutamate challenge (5 mM). After 12 h, the apoptotic bodies and mitochondrial membrane potential in the harvested cells were analyzed by flow cytometry-indicated absence.