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. 2020 Sep 12;21(18):6687. doi: 10.3390/ijms21186687

Figure 9.

Figure 9

PCS induced neuroinflammation. Unilateral nephrectomized mice were intraperitoneally injected with PCS (0, Control; and 100 mg/kg, PCS) and the PCS-injected mice were orally given with AST-120 (400 mg/kg, PCS/AST-120) for 7 weeks. (A) The serum samples were collected and subjected to the measurement of IL-1β levels. (B) Total RNAs were extracted from the isolated prefrontal cortical tissues and subjected to quantitative RT-PCR for the measurement of IL-1β mRNA levels. Proteins were extracted from the isolated prefrontal cortical tissues and subjected to a Western blot test with the indicated antibodies. Representative blots (C) and the quantitative data (D) are shown. Nuclear proteins were extracted from the isolated prefrontal cortical tissues and subjected to EMSA for the measurement of NF-κB and AP-1 DNA-binding activity. Representative blots (E) and the quantitative data (F) are shown. * p < 0.05 vs. control group and # p < 0.05 vs. PCS group, n = 8.