Figure 2.

(a) A schematic illustration of the SAXS experiment (left) and a photograph of the specimen chamber (right). In the right photograph, the phot2 specimen was photo-excited by BL irradiation of a photo-diode. (b) SAXS profile of protein disulfide isomerase (PDI) from a thermophilic fungus [44]. A model of PDI is shown in the inset. The enhancements at approximately S = 0.03 Å⁻¹ are caused by the characteristic arrangement of the four domains in PDI. (c) A Guinier plot for the small-angle region in the SAXS profile in panel (b). The dashed regression line indicates Rg of 33.4 ± 0.4 Å. (d) Schematic illustration of the multivariate analysis of models restored by ab initio calculation by the GASBOR program [45]. The details are described in the Materials and Methods section.