Skip to main content
. 2020 Sep 10;21(18):6631. doi: 10.3390/ijms21186631

Figure 2.

Figure 2

Genotoxicity of BrdU and EdU. (a) Hypoxanthine phosphorybosyl transferase (HPRT) mutation induction with BrdU and EdU; (b) chromosomal aberration formation in genomic instability 24 h after completing BrdU or EdU treatment; (c) endoreduplication formation after BrdU and EdU uptake. White bar indicates control. Black bar indicates BrdU treatment. Grey bar indicates EdU treatment. Error bars represent the standard error of the mean of three independent experiments. One-way ANOVA, Dunnett’s Multiple Comparison Test was performed to provide p-values. * indicates statistically significant differences (p < 0.05); (d) a representative image of CHO metaphase spread for control; (e) a representative image of genomic instability after EdU treatment. Red arrows indicate breaks and blue arrows indicate exchanges; (f) a representative image of EdU-induced endoreduplication. Red arrows indicate endoreduplicated chromosomes.