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. 2020 Sep 7;21(18):6536. doi: 10.3390/ijms21186536

Figure 1.

Figure 1

Curing of [PSI+] in SY80 yeast strain by overexpression of dominant negative Hsp104 mutant. (A) Time course of curing [PSI+] as a function of generation time. (B) Images of the green fluorescent labeled (GFP)-labeled foci in SY80 yeast strain before induction, after 5 generations in galactose medium to induce expression of the dominant negative mutant, and completely cured. SY80 strain contains a relatively strong [PSI+] variant, which was engineered to express GFP-labeled Sup35 from the SUP35 chromosomal locus [26].