Skip to main content
. 2020 Sep 7;21(18):6536. doi: 10.3390/ijms21186536

Figure 3.

Figure 3

Curing of [PSI+] in SY80 yeast strain by overexpression of a S. pombe Hsp104 fragment, Hsp104ΔC32. (A) Time course of curing [PSI+] as a function of generation time. (B) Images of the GFP-labeled Sup35 in SY80 before induction and after inducing S. pombe Hsp104ΔC32 expressing for 2 generations with galactose. (C) Flow cytometry analysis of curing of [PSI+] in SY80 strain expressing S. pombe Hsp104ΔC32 for 6 generations. Cell wall was fluorescently labeled with wheat germ agglutin, as described previously [34], mother cells were then separated from progeny based on fluorescent labeling by flow cytometry.