Figure 4.

Curing of [PSI⁺] by overexpression of dominant negative Hsp104 mutant and wild-type Hsp104 in different strains. Curing by overexpression of dominant negative mutant is shown by solid symbols and solid line, while curing by overexpression of wild-type is shown by open symbols and dashed line. (A) Comparison of curing by overexpression of dominant negative mutant (from Figure 1A) and wild-type Hsp104 (from Figure 2A) were obtained using relatively strong [PSI⁺] variant in the SY80 yeast strain, a relatively strong variant. (B) Comparison of curing by overexpression of dominant negative mutant and wild-type Hsp104 were obtained using the relatively weak [PSI⁺] variant in the L2888 yeast strain from the Liebman laboratory [43]. (C) Comparison of curing by overexpression of dominant negative mutant and wild-type Hsp104 were obtained using a relatively strong [PSI⁺] variant in 779-6a yeast expressing red fluorescent protein (RFP)-labeled Sup35 instead of Sup35 from the SUP35 chromosomal locus.