Figure 8.

Nitrite quantification in supernatant of BALB/c peritoneal macrophages treated with 1,4-disubstituted-1,2,3-triazole compounds. Cells are stimulated (or not stimulated) with Leishmania amazonensis and treated for 48 h with compound 2, 4, or 5 at 37.5 μM, or with compound 6 at 18.7 μM. Data represents mean ± SD of at least three independent experiments realized at least in quadruplicate. ** p = 0.0039, *** p = 0.0001 when compared to untreated and non-infected cells, or between brackets by Kruskal-Wallis followed by Dunn’s multiple comparisons test.