Video 3.
Overexpressed NuMA-FL-EGFP can form stable cable-like structures in the nucleus. Time-lapse imaging of a representative RPE1 cell transiently overexpressing NuMA-FL-EGFP imaged before and during FRAP. The red circle highlights the photobleached area. Fluorescence recovery in the bleached area is minimal, consistent with NuMA-FL being able to form higher-order structures in the nucleus. Time in minutes:seconds, with the frame corresponding to bleaching set to 00:00. Scale bar, 5 µm. Video corresponds to the still images in Fig. 3 G.