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. 2020 Sep 10;21(18):6640. doi: 10.3390/ijms21186640

Figure 2.

Figure 2

cAMP treatment represses autophagy rate and flux. Adipocytes were differentiated and treated as in Figure 1. Autophagy related (ATG) 5, ATG7 and ATG12 gene expression (A) in primary and (B) SGBS cells normalized to GAPDH, quantified by RT-qPCR (n = 5). Representative immunoblots and densitometry analysis of LC3-II/LC3-I protein expression ratio (left) and p62 protein expression (right) normalized to β-actin (C) in hASC-derived and (D) in SGBS cells (n = 5). Results are expressed as mean ± SD. Statistics: two-tailed paired student t-test; * p < 0.05; ** p < 0.01; * significant as compared to white untreated sample; △ p < 0.05; △△ p < 0.01; △ significant as compared to beige untreated sample.