WHC disturbed cell cycle progression of breast cancer cells. (A,B) Cell cycle profiles and statistics for dose effects of WHC. Breast cancer (MCF7 and SKBR3) cells were treated with WHC (control (0.075% DMSO), 0.25, 0.5, and 0.75 μM, respectively) for 48 h. (C,D) NAC pretreatments reversed the WHC induced cell cycle disturbance. Following pretreatments with NAC (10 mM for 1 h), cells were treated with the control (0.075% DMSO) and 0.75 μM of WHC for 0, 24, and 48 h, i.e., NAC/WHC. Data are means ± SDs (n = 3). Results marked without overlapping letters show significant differences (P < 0.05 to 0.0001).