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Spatial transcriptomics. Briefly, fresh frozen tissue is cryo-sectioned and mounted into a slide containing barcoded spots that provide a specific spatial orientation. The cells are permeabilized and the mRNA binds to the most proximal barcode, correlating single-cell transcriptomics with a spatial tissue location. This technique is also compatible with the different sc-RNA-seq analysis shown in Figure 4. Image created with BioRender.com.
