Table 2.
Single-cell -omics research in gonadal development, differentiation and maturation.
| Year | Authors | Organism | Developmental Stage | Tissue | Technology | Cells | Data |
|---|---|---|---|---|---|---|---|
| 2015 | Guo et al. [116] | Human embryo (male and female) | Males: 4w, 7w, 10w, 11w and 19w. Females: 4w, 8w, 10w, 11w and 17w |
PGCs and somatic cells | MACS, FACS, Tang et al. [117] | 319 | GSE63818 |
| 2015 | Lindeman et al. [118] | CAG-Stop-Dmrt1-Gfp; Sf1-Cre mice (female) | P17 | GFP sorted supporting cells | FACS, SMARTer (C1) | 68 | GSE64960 |
| 2017 | Li et al. [119] | Human embryo (male and female) | Males: 4w, 9w, 10w, 12w, 19w, 20w, 21w and 25w. Females: 5w, 7w, 8w, 10w, 11w, 12w, 14w, 18w, 20w, 23w, 24w and 26w |
PGCs and somatic cells | MACS, FACS and Smart-seq2 | 2167 | GSE86146 |
| 2018 | Chen et al. [120] | Tg(Vasa-dTomato; Lin28-YFP) mice (male) | Unspecified | Spermatogenic cells | FACS, modified Smart-seq2 | 1136 | GSE107644 |
| 2018 | Green et al. [121] | C57BL/6J mice, Gfra1CreERT2; RosamT/mG, Amh-cre; RosamT/mG and Sox9-eGFP mice (male) | Adult | Whole testis, interstitial cells, spermatogonia and Sertoli cell enrichment, haploid depletion. | FACS, Drop-Seq | 34,644 | GSE112393 |
| 2018 | Guo et al. [114] | Human (male) | Adult (17, 24 and 25 yo) and infant (13 months old) | Whole testis | Chromium | 7790 | GSE120508 |
| 2018 | Hermann et al. [122] | Human and mice (male) | Human adults (mean = 41.6 yo). Mouse P6 and adults | Spermatogenic cells | FACS, Chromium & SMARTer (C1) | 62,000 | GSE108970, GSE108974, GSE108977, GSE109049, GSE109033 and GSE109037 |
| 2018 | Stévant et al. [21] | Tg(Nr5a1-GFP) mice (male) | E10.5, E11.5, E12.5, E13.5 and E16.5 | Nr5a1+ gonadal somatic cells | FACS, SMARTer (C1) | 400 | GSE97519 |
| 2018 | Vértesy et al. [123] | Human (female) | 8 to 14.4 weeks of development | Whole fetal gonads and adrenal germ cells | Smart-seq2 | 108 | GSE79280 |
| 2018 | Wang et al. [124] | Human adult (male) | Normal: 30 and 60 yo. Obstructive azoospermia: 27, 29, 34, 39, 41, 43 and 44 yo |
Whole testis | Smart-seq2 | 2854 | GSE106487 |
| 2018 | Zhang et al. [125] | Human (female) | 24 to 32yo | Oocytes and granulosa cells | Tang et al. [117] | 151 | GSE107746 |
| 2019 | Ernst et al. [126] | C57BL/6J and TC1 mice (male) | P5, P10, P15, P20, P25, P30, P35, Adult (8–9 weeks) | Whole testis | Chromium | 53,510 | E-MTAB-6946 |
| 2019 | Fan et al. [127] | Human (female) | Adult | Ovarian inner cortex | Chromium | 20,676 | GSE118127 |
| 2019 | Grive et al. [128] | B6D2F1/J mice (male) | Postnatal days 6, 14, 18, 25, 30 and 8 weeks old | Whole testis, spermatogonia cells enrichment. | MACS, Chromium | 15,493 | GSE121904 |
| 2019 | Gu et al. [129] | ICR Mouse (female) | 14 days old | Mouse ES cells, MII oocytes and growing oocytes | scCOOL-seq [130] and Tang et al. [117] | 1070 | GSE114822 |
| 2019 | Law et al. [131] | Blimp1-CreTg;Id4-eGFPTg;Rosa26tdTomato-fl_STOP_fl/LacZ mice (male) | E16.5, P0, P3 and P6 | Germ cells | FACS, Chromium | 10,140 | GSE124904 |
| 2019 | Sohni et al. [132] | Human (male) | Neonatal (2 and 7 days old) and adult (37 and 42 yo) | Whole testis | Chromium | 33,585 | GSE124263 |
| 2019 | Stévant et al. [133] | Mouse Tg(Nr5a1-GFP) (female) | E10.5, E11.5, E12.5, E13.5, E16.5 and P6 | Nr5a1+ gonadal somatic cells | FACS, SMARTer (C1) | 563 | GSE119766 |
| 2020 | Estermann et al. [134] | Chicken embryo (male and female) | E4.5, E6.5, E8.5 and E10.5 | Whole gonads | Chromium | 33,247 | GSE143337 |
| 2020 | Guo et al. [115] | Human (male and transfemale) | Male: 7, 11, 13 and 14 yo. Transfemale: 26 and 50 yo |
Whole testis | Chromium | 9836 | GSE134144 |
| 2020 | Lau et al. [135] | Macaque (male) | Infant (1 yo), Juvenile (2 yo) and Adult (4 yo) | Whole testis and sorted spermatogonia cells | FACS, Chromium | 16,932 | E-MTAB-8979 |
| 2020 | Niue et al. [34] | Mice (female) | E11.5, E12.5, E14.5, E16.5, E18.5, P1 and P5 | Whole Ovary | Chromium | 52,542 | GSE136441 |
| 2020 | Shami et al. [136] | Human and Macaque (male) | Adult human (20–40 yo), Adult Macaque (4–13 yo) | Whole testis | Drop-seq | 36,000 | GSE142585 |
| 2020 | Tan et al. [137] | C57BL/6 mice (male) | E18.5, P2 and P7 | Whole testis | Chromium | 50,859 | GSE130593 |
| 2020 | Wagner et al. [138] | Human adult (female) | 20–38 yo | Ovarian cortex and DDX4 sorted cell | FACS, Chromium and Smart-seq2 | 24,329 | E-MTAb−8381, E-MTAb−8403 |
| 2020 | Wang et al. [139] | Macaque (female) | Juvenile (4–5 yo) and Aged (18–20 yo) | Whole ovaries | STRT-seq | 2601 | GSE130664 |
| 2020 | Xia et al. [140] | C57BL/6J mice and human (male) | Human adult (40 and 45 yo); 4 months old mice | Whole testis, germ cell enrichment | InDrops | 4147 | GSE125372 |
Abbreviations: E: embryonic day, P: postnatal day, w: weeks, yo: years old.