Figure 3.

siRNA knockdown of FKBP12 enables ALK2^WT to mediate the activin A-induced BMP signaling in C2C12BRA cells. (A) Specific siRNA (siFKBP) effectively knocked down FKBP12 in the stably transfected BMP-responsive C2C12BRA cells (siCtr: scrambled siRNA). (B) Luciferase assay result indicated that FKBP12 knockdowns induced activin A-induced BMP signaling, whereas the overexpression of FKBP12 suppressed the activin A-induced BMP signaling in C2C12BRA cells. (C) Western blotting confirmed that FKBP12 knockdowns induced activin A-induced BMP signaling. Results of luciferase assay and RT-PCR assay were represented as the mean relative luciferase units (RLU)  ±  SEM and relative quantity (RQ) ± SEM (n  =  3, * p < 0.05, ** p < 0.01).