Figure 2.

The effect of Src and Pyk2 inhibition on αIIbβ3-mediated PKCδ phosphorylation. (A) Fibrinogen-adherent platelets in the presence of 10 µM PP2 or 10 µM PP3 were probed with anti-phospho-PKCδ (Tyr311) or anti-β-actin antibodies. The blots shown are representative of three independent experiments and are presented as mean ± SE. * p < 0.05. (B) Fibrinogen-adherent platelets in the presence of Pyk2 inhibitor TAT-Pyk2-CT or TAT-GFP (control) were probed with anti-phospho-PKCδ (Tyr311) or anti-β-actin antibodies by Western blotting. The blots shown are representative of three independent experiments and are presented as mean ± SE. * p < 0.05.